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Confluent and subconfluent HUVEC, grown on coverslips, were fixed and subjected to confocal fluorescent microscopy using specific antibodies to (A) CAV1 and (B) SNAP-23 in comparison with eNOS. Note the clear spatial separation of CAV1 in subconfluent cells from the similar localization patterns of SNAP-23 and eNOS at the PM. Scale bars represent 10 μm.
