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. 2006 Nov 28;400(Pt 3):467–476. doi: 10.1042/BJ20060437

Figure 4. Effect of increasing quantities of greglin on the activity of 18 nM PPE.

Figure 4

The residual enzyme activity was measured with Suc-(Ala)3-p-NA as indicated in the text. Ki(app), the substrate-dependent Ki, was obtained by fitting the experimental equilibrium titration data to eqn (3) [16]:
graphic file with name M3.gif (3)
where a is the enzyme relative activity as defined in the legend of Figure 3. [E]0 and [I]0 are the initial enzyme and inhibitor concentrations respectively. The theoretical curve was generated with Ki(app)=0.102 μM.