Table 2. Apparent and real dissociation constants for PrP91–115.
pH | Buffer | Kd(1) (app) | Kd(1) (real) | Kd(2) (real) | Kd(3) (app) | Kd(3) (real) |
---|---|---|---|---|---|---|
7.4 | 5 mM Tris | 30±10 μM | 100±50 nM | 5±1 μM | 40±10 μM | 140±50 nM |
5.5 | 10 mM Acetate | 1±0.1 mM | 300±100 μM | 40±10 μM | − | − |
7.4 | 50 mM Mops | 6±1 μM | 250±30 nM | − | 6±1μM | 250±30 nM |