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. 2006 Nov 28;400(Pt 3):477–484. doi: 10.1042/BJ20061103

Figure 3. Coomassie-stained SDS/PAGE of gel filtration fractions.

Figure 3

Fractions from a gel filtration experiment with light-treated and dark-treated His-tagged R. capsulatus H-subunit were analysed by SDS/PAGE; 10 μg of protein was loaded. Lane 1, size marker; lane 2, pooled fractions 7–12 of the dark-treated sample in Figure 2(A); lane 3, pooled fractions 18–27 of the dark-treated sample; lane 4, pooled fractions 7–12 of the light-exposed sample; lane 5, pooled fractions 18–27 of the light-exposed sample. The solid and dashed arrows indicate His-tagged H-subunit and high-molecular-mass aggregates retarded in the stacking gel respectively.