Figure 4. Inhibition of sulfation and heparinase III digestion using purified virus preparations.

(A) Transduction rate assay of the effect of incubating producer cells in 35 mM sodium chlorate (NaClO₃) with or without subsequent low-speed centrifugation for purification of virus particles from soluble factors in the conditioned medium. Purified virus was first diluted to normalize CA content compared with the unpurified preparation. The initial transduction rate is expressed relative to that of the unpurified control virus. There was no significant difference in the effect of NaClO₃ with/without purification (P>0.05, one-way ANOVA pairwise). Results shown are means±S.E.M. values (n=3). (B) Transduction rate assay of the effect of digesting virus, with or without prior purification, for 4 h at 37 °C with the indicated concentrations of heparinase III. Purified virus was first diluted to normalize CA content compared with the unpurified preparation. The initial transduction rate is expressed relative to that of the unpurified control virus. There was no significant difference in the effect of digestion at any concentration (P>0.05, one-way ANOVA pairwise). Results shown are means±S.E.M. values (n=3).