Skip to main content
. 2006 Nov 28;400(Pt 3):573–582. doi: 10.1042/BJ20060831

Figure 1. Assaying for transcriptional regulatory domains encoded by TopBP1.

Figure 1

(a) Fusion proteins between the TopBP1 sequences shown and the Gal4 DBD were constructed in order to assay for transcriptional-control domains of intact TopBP1 and the regions shown. To the right-hand side of the Figure is the predicted size of the fusion proteins in kDa (kD). Boxes 1 to 8 represent the BRCT domains. (b) The plasmids encoding the proposed fusion proteins described in (a) were transfected into HEK-293T cells. Protein extracts were prepared from these cells and Western blots using an anti-Gal4 antibody were performed. A representative blot is shown. The numbers to the left-hand side of the blot represent the marker proteins sizes in kDa. (c) The ability of these proteins to activate transcription from a Gal4 DNA-binding site-containing promoter, pG5luc, was assayed in HEK-293T cells. The results are expressed relative to the Gal4 DBD-expressing plasmid pBIND.