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. 2006 Nov 28;400(Pt 3):573–582. doi: 10.1042/BJ20060831

Figure 2. TopBP1 encodes a transcriptional-activation domain.

Figure 2

(a) Further deletion mutants of TopBP1 were prepared as shown and to the right-hand side of the Figure is the predicted size of the fusion proteins in kDa (kD). (b) The ability of these proteins to activate transcription from a Gal4 DNA-binding site-containing promoter, pG5luc, was assayed in HEK-293T cells. The results are expressed relative to the Gal4 DBD-expressing plasmid pBIND. (c) The plasmids encoding the proposed fusion proteins described in (a) were transfected into HEK-293T cells. Protein extracts were prepared from these cells and Western blots using an anti-Gal4 antibody were performed. A representative blot is shown. The numbers to the left-hand side of the blot represent the marker proteins sizes in kDa.