Skip to main content
. 2006 Nov 28;400(Pt 3):573–582. doi: 10.1042/BJ20060831

Figure 3. The TopBP1 repressor domain is able to repress the strong transcriptional activation domain of VP16.

Figure 3

(a) The diagram is a representation of the plasmids encoding Gal4 DBD fusion proteins with the VP16 activation domain with the TopBP1 repressor domain or without. (b) The ability of these proteins to activate transcription from a Gal4 DNA-binding site-containing promoter, pG5luc, was assayed in HEK-293T cells. The results are expressed relative to the Gal4 DBD-expressing plasmid pBIND. (c) The plasmids encoding the proposed fusion proteins described in (a) were transfected into HEK-293T cells. Protein extracts were prepared from these cells and Western blots using an anti-Gal4 antibody were carried out. A representative blot is shown. The numbers to the left-hand side of the blot represent the marker proteins sizes in kDa. (d) Similar experiments to those described in (b) were carried out with the samples indicated pre-treated with TSA prior to cell harvest. In this figure the results are shown as relative light units (RLU) per μg of protein used in the luciferase assay.