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. 2006 Nov 28;400(Pt 3):573–582. doi: 10.1042/BJ20060831

Figure 4. The TopBP1 activation domain maps to a region between amino acids 460 and 500, incorporating the N-terminal part of BRCT4.

Figure 4

(a) A schematic representation of Gal4 DBD fusion proteins investigated for their ability to activate transcription. (b) The ability of these proteins to activate transcription from a Gal4 DNA-binding site containing promoter, pG5luc, was assayed in HEK-293T cells. The results are expressed relative to the Gal4 DBD-expressing plasmid pBIND. (c) The plasmids encoding the proposed fusion proteins described in (a) were transfected into HEK-293T cells. Protein extracts were prepared from these cells and Western blots using an anti-Gal4 antibody were performed. A representative blot is shown. The numbers to the left-hand side of the blot represent the marker proteins sizes in kDa. (d) An alignment of the human TopBP1 transcriptional-activation domain between amino acids 460 and 500 with that of other species. Of note are the clusters of hydrophobic residues. The closed circles (●) above the sequence indicates hydrophobic residues, the asterisk (*) highlights identical residues, the colon (:) conserved substitutions and the period (·) semi-conserved substitutions.