Figure 10.—

Dependence of ilr3 IAA–Leu phenotypes on the metal environment. (A) Root lengths of wild-type Col-0, the amidohydrolase mutant ilr1-5, the gain-of-function ilr3-1 allele, and the loss-of-function ilr3-2 allele grown for 8 days at 22° with continuous illumination under yellow filters on media containing 0.5% sucrose and various concentrations of Fe–EDTA. (B) The plant types from A were grown on media containing 0.5% sucrose and various concentrations of Fe–EDTA with 20 μm IAA–Leu. Points represent means plus or minus standard errors of the means (n ≥ 6). ilr3-1 roots were significantly longer than wild type on 20 μm IAA–Leu supplemented with 5, 30, and 90 μm Fe–EDTA, and ilr3-2 roots were significantly shorter than wild type on 20 μm IAA–Leu supplemented with 2 and 5 μm Fe–EDTA (P ≤ 0.001 in two-tailed t-tests assuming unequal variance).