Figure 4.—

Altered ilr3-1 responses to IAA conjugates and metals. (A) ilr3-1 responses to IAA–amino acid conjugates. Root lengths of wild-type Col-0 (Wt), ilr3-1, ilr1-5, iar3-1, and iar1-3 seedlings grown for 8 days on unsupplemented medium with 0.5% sucrose or medium containing 20 μm IAA–Leu, IAA–Phe, or IAA–Ala and 0.5% sucrose are shown. (B) ilr3-1 has wild-type responses to free auxins. Root lengths of Col-0 (Wt) and ilr3-1 seedlings grown for 8 days on medium without hormones or media containing 10 nm IAA, 10 μm IBA, 300 nm NAA, or 60 nm 2,4-D and 0.5% sucrose are shown. (C) Root lengths of Col-0 (Wt) and ilr3-1 seedlings grown for 8 days in yellow-filtered light at 22° on medium without sucrose containing no supplemental metal, 2 mm MnCl₂, or 100 μm CoCl₂. Error bars indicate standard errors of the means (n ≥ 10). Two-tailed t-tests assuming unequal variance were performed to compare mutant and wild-type values; *, P < 0.01; **, P < 0.001.