TABLE 1.

Gene expression in 7-day-old ilr3-1 mutant and wild-type seedlings

		Microarray			qRT–PCR:
Signala		Fold changeb:	Fold changec:
Transcript	Encoded protein	ilr3-1	Wt	ilr3-1/Wt	ilr3-1/Wt
At5g54680	ILR3 bHLH–leucine zipper transcription factor	168	2122	−12.6*	−4.4 ± 0.7
At5g51720	Expressed protein (zinc-finger domain)	94	1226	−13.0*	−37.4 ± 4.8
At1g76800	Putative metal transporter (Ccc1p-like)	56	267	−4.4*	−5.7 ± 0.2
At3g25190	Putative metal transporter (Ccc1p-like)	662	2050	−2.9*	−5.7 ± 0.3
At1g21140	Putative metal transporter (Ccc1p-like)	189	525	−2.7*	−4.1 ± 0.5
At3g12900	Oxidoreductase-like	284	946	−3.2*	1.0 ± 0.5
At3g02875	ILR1 IAA–Leu hydrolase	107	145	−1.3*	−1.3 ± 0.3
At1g51760	IAR3 IAA–Ala hydrolase	ND	ND	ND	−1.4 ± 0.2
At3g18485	ILR2 novel protein	ND	ND	ND	−1.1 ± 0.04
At1g68100	IAR1 ZIP-like metal transporter	793	769	−1.0	ND
At1g24180	IAR4 pyruvate dehydrogenase E1α	1737	1813	1.1	ND

^aMean signal from three biological replicates. ND, not determined. (Probes for IAR3 and ILR2 are not present on the Affymetrix ATH1 chips.)

^bAsterisks indicate fold changes that are significant (P < 0.05) according to two-tailed t-tests assuming unequal variance.

^cError represents the standard deviation of the fold change values from three PCR replicates of each of two biological replicates.