Figure 1.—

The cdc53-1 and cdc34-2 mutants display cell integrity defects. (A) The cdc53-1 and cdc34-2 mutants display phenotypes consistent with cell integrity defects. Wild-type (WT), cdc53-1, and cdc34-2 cells were grown on YPD or YPD containing 1 m sorbitol, 0.5 m NaCl, 0.0050% SDS, or 0.0075% SDS. The growth of the cells incubated at 30°, 33°, 35°, and 37° is shown. (B) cdc53-1 and cdc34-2 mutants grown in the presence of 1 m sorbitol display morphological and cell cycle defects. Cultures of cdc53-1 and cdc34-2 cells were grown in YPD media or YPD media containing 1 m sorbitol at 30° until midlog phase. These cells were shifted to 37° and samples were taken from each temperature and examined for morphology by microscopy and for DNA content as shown. (C) Deletion of SIC1 does not suppress the cdc34-2 and cdc53-1 cell integrity defects. sic1Δ, cdc34-2 sic1Δ, and cdc53-1 sic1Δ cells were grown on YPD and YPD containing 1 m sorbitol or 0.0050% SDS. The growth of the cells incubated at 30°, 33°, 35°, and 37° is shown.