TABLE 3.
Segregation in progeny of double-introgression lines containing two S. lycopersicoides segments on the same chromosome pair, oriented in repulsion phase, and corresponding single-segment control lines
| Genotypic classesb
|
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chr. | Cross (♀ × ♂)a | Line | A +/A + | A +/+ B or + +/A B | + B/+ B | + +/+ + | + +/A + | + +/+ B | A B/A + | A B/+ B | A B/A B | nc | Exp. ratio | χ2d |
| 1 | Control F2 (1A/+) × self | LA4296 | 20 | 16 | 53 | 89 | 1:2:1 | 3.60 (NS) | ||||||
| 1 | Control F2 (1B/+) × self | LA4233 | 1 | 6 | 45 | 52 | 1:2:1 | 28.7*** | ||||||
| 1 | F2 (1A +/+ 1B) × self | 3 | 11 | 3 | 1 | 10 | 5 | 2 | 8 | 0 | 43 | 1:2:1 | 1.47 (NS) | |
| 1 | BC (1A +/+ 1B) × + + | 0 | 47 | 39 | 42 | 128 | 1:1 | 0.049 (NS) | ||||||
| 2 | Control F2 (2A/+) × self | LA4237 | 13 | 32 | 49 | 94 | 1:2:1 | 7.85*** | ||||||
| 2 | Control F2 (2B/+) × self | LA4239 | 16 | 44 | 52 | 112 | 1:2:1 | 14.6*** | ||||||
| 2 | F2 (2A +/+ 2B) × self | 2 | 25 | 9 | 1 | 15 | 13 | 5 | 11 | 0 | 81 | 1:2:1 | 6.52* | |
| 2 | BC (2A +/+ 2B) × + + | 17 | 23 | 20 | 36 | 96 | 1:1 | 4.02* | ||||||
| 2 | BC + + × (2A +/+ 2B) | 24 | 33 | 51 | 67 | 175 | 1:1 | 1.91 (NS) | ||||||
| 7 | Control F2 (7A/+) × self | LA4261 | 4 | 43 | 17 | 64 | 1:2:1 | 61.6*** | ||||||
| 7 | Control F2 (7B/+) × self | LA4259 | 20 | 24 | 52 | 100 | 1:2:1 | 1.00 (NS) | ||||||
| 7 | Control F2 (7C/+) × self | LA4258 | 37 | 31 | 41 | 109 | 1:2:1 | 7.35*** | ||||||
| 7 | F2 (7A +/+ 7B) × self | 0 | 8 | 18 | 3 | 4 | 15 | 0 | 5 | 0 | 53 | 1:2:1 | 28.8*** | |
| 7 | F2 (7A +/+ 7C) × self | 1 | 19 | 16 | 10 | 6 | 44 | 2 | 17 | 3 | 118 | 1:2:1 | 12.6** | |
| 7 | BC + + × (7A +/+ 7B) | 15 | 30 | 14 | 82 | 141 | 1:1 | 46.8*** | ||||||
| 7 | BC + + × (7A +/+ 7C) | 9 | 29 | 9 | 40 | 87 | 1:1 | 18.4*** | ||||||
The introgressed segments in each line are shown on the maps in Figure 4, where A denotes the segment on the short arm and B the segment on the long arm of the same chromosome. The 7C segment on chromosome 7 is considered the B segment. The wild-type or L. esculentum alleles at the corresponding marker loci are indicated by a +. Note that the control, single-segment populations were grown separately.
Segregation data are the number of plants in each genotypic class; underlined values indicate genotypes with crossovers between the A and B segments.
n is the sample size, excluding individuals with recombination within the A or B segments.
Chi-square values test for goodness-of-fit to expected Mendelian ratios and are based on data in the parental (nonrecombinant) classes only. Significance levels are: NS, not significant; *P < 0.05, **P < 0.01, and ***P < 0.001.