Table 1. The frequencies of the resonance Raman modes of the 6C FeIII–NO complexes of NOSs.
The spectra of saNOS were acquired over a very short time (5 min) as the sample autoreduced over time to form a FeII–NO complex. −, absent; Arg, L-arginine; ND, not determined; sh, shoulder.
| Protein | νFe−NO (15N16O) cm−1 | δFe−N−O (15N16O) cm−1 | Reference |
|---|---|---|---|
| saNOS | 539 (Δ4) | 551 (Δ∼16), sh | This work |
| saNOS–H4B | 539 (Δ3) | 551 (Δ∼16), sh | This work |
| saNOS–THF | 539 (ND) | ND | This work |
| saNOS–Arg | – | 541 (Δ7) | This work |
| saNOS–Arg–H4B | – | 542 (Δ8) | This work |
| saNOS–NOHA–H4B | – | 543 (ND) | [20] |
| saNOS–citrulline–H4B | – | 549 (Δ12) | [20] |
| bsNOS | 540 (Δ3) | 551 (Δ∼16), sh | This work, [33] |
| bsNOS–H4B | 540 (ND) | ND | This work, [33] |
| bsNOS–THF | 540 (ND) | ND | This work |
| bsNOS–Arg | – | 541 (Δ7) | This work, [33] |
| bsNOS–Arg–H4B | – | 542 (Δ8) | This work, [33] |
| bsNOS–NOHA–H4B | – | 542 (Δ7) | This work |
| bsNOS–citrulline–H4B | – | 547 (Δ9) | This work |
| nNOSox | 535 (Δ3) | – | [40] |
| nNOSox–H4B | 542 (Δ5) | – | [40] |
| nNOSox–Arg–H4B | – | 546 (Δ10) | [40] |
| iNOSox | 537 (Δ4) | – | [41] |
| iNOSox–H4B | 541 (Δ4) | 550 (Δ13) | [41] |
| iNOSox–Arg | 537 (Δ4) | – | [41] |
| iNOSox–Arg–H4B | – | 545 (Δ8) | [41] |