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. 2006 Dec 11;401(Pt 1):325–331. doi: 10.1042/BJ20060648

Figure 2. Formation of SDS-stable complexes between recombinant Spn4E and Dfurin1 or Dfurin2.

Figure 2

Supernatants (20 μl) from S2 cells were incubated in the absence (lanes 1 and 3) or presence (lanes 2 and 4) of 50 ng of recombinant Spn4E. After reducing SDS/PAGE (10% gels), the reaction products were analysed by Western blotting, using antibodies directed against Dfurin1 (lanes 1 and 2) or Dfurin2 (lanes 3 and 4) respectively. An asterisk marks Spn4E–Dfurin1 and Spn4E–Dfurin2 complexes depicting the expected sizes. The molecular masses of marker proteins are indicated on the right.