Table 1. Inhibitory profile of Spn4 isoforms.
The association rate constants kassoc and the SI values were determined as described in the Experimental section. –, no inhibition.
| Spn4E | Spn4F | Spn4G | Spn4H | |||||
|---|---|---|---|---|---|---|---|---|
| Enzyme | kassoc (M−1·s−1) | SI | kassoc (M−1·s−1) | SI | kassoc (M−1·s−1) | SI | kassoc (M−1·s−1) | SI |
| Furin (human) | (5.5±0.5)×106* | 3.5* | – | – | – | |||
| Neutrophil elastase | – | (4.6±0.4)×104 | >4‡ | (3.9±0.2)×104 | >4‡ | (7.6±0.9)×104 | >4‡ | |
| Cathepsin L | – | (1.0±0.1)×106 | 1.0 | (2.6±0.8)×105 | 4.3 | – | ||
| Cathepsin S | – | (1.8±0.4)×105 | 2.7 | (9.2±0.8)×104 | 3.6 | – | ||
*Data from [12].
‡Estimated from Western blotting experiments.