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. 2006 Dec 11;401(Pt 1):65–78. doi: 10.1042/BJ20060732

Figure 7. Different effects of JNK isoforms on PAR-1-dependent c-Jun activation in rat astrocytes.

Figure 7

Cells, transfected with (A1 and A2) JNK1 siRNA 1 (J1 siRNA, 100 nM), (B1 and B2) JNK2 siRNA 1 (J2 siRNA, 100 nM) or (C1 and C2) JNK3 siRNA 1 (J3 siRNA, 100 nM) for 48 h, were treated with 10 μM TRag or without in serum-free medium for 1 h. Untransfected cells served as control (C), non-transfected cells stimulated with 10 μM TRag for one hour were regarded as positive control. (A1, B1 and C1) show representative blots for c-Jun phosphorylation (p-c-Jun, upper panel) and β-tubulin (lower panel). (A2, B2 and C2) show the relative values of c-Jun phosphorylation normalized to β-tubulin. Results are presented as means±S.E.M. for at least three independent experiments. ***P<0.001, **P<0.01, *P<0.05 as compared with the control cells.