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. 2006 Dec 11;401(Pt 1):269–277. doi: 10.1042/BJ20060890

Figure 1. Inhibition of IGF-I binding to the IGF-1R by anti-IGF-1R mAbs.

Figure 1

Increasing concentrations of mAbs 7C2 (●), 9E11 (▽) and 24-60 (□) and a control IgG1 (◆) were tested for their ability to inhibit Eu–IGF-I (A) and Eu–IGF-II (B) binding to immunocaptured IGF-1R from solubilized P6 cell extracts. IGF-I and IGF-II (10 nM) were also used in the competition assay. Results are expressed as a percentage of europium label bound in the absence of competing mAb or ligand. The P values were calculated by comparing the data for no treatment (buffer) with other data.