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. 2006 Dec 11;401(Pt 1):269–277. doi: 10.1042/BJ20060890

Figure 4. Effect of IGF-I, IGF-II and chimaeras on binding of s-IGF-1R to mAb 9E11.

Figure 4

BIAcore analysis was used to detect the effect of (A) IGF-I, (B) IGF-II CI, (C) IGF-II or (D) IGF-I CII on the association and dissociation of s-IGF-1R with captured mAb 9E11. Increasing concentrations of ligands (from top to bottom, 0, 0.1, 1, 10 and 100 nM) were pre-incubated with s-IGF-1R prior to injection over a mAb 9E11 sensor surface. IGFs and chimaeric ligands were incubated with 12.5 or 6.25 nM of s-IGF-1R respectively. Each panel is representative of two or three experiments.