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. 2006 Dec 11;401(Pt 1):269–277. doi: 10.1042/BJ20060890

Figure 6. Inhibition of MCF-7 breast cancer cell migration and stimulation of IGF-1R down-regulation by anti-IGF-1R mAbs.

Figure 6

(A) MCF-7 cell migration towards increasing concentrations of IGF-I was measured in the presence of 25 nM mAbs 7C2, 9E11, 24-60 and αIR-3 or a control IgG1. P values represent significance compared with no mAb at the matched concentration (*0.01<P<0.05; ¶ 0.001<P<0.01). Results are means±S.D. of triplicates and a representative of three separate experiments is shown. (B) Immunoblot of MCF-7 cell lysates from untreated cells or MCF-7 cells treated with IGF-I, 9E11, 7C2, 24-60 or αIR-3 and R− cell lysates probed with anti-IGF-1R C-20. Bands represent (a) pro-IGF-1R, (b) IGF-1R β subunit and (c) a non-specific band (used here as loading control).