Figure 1. Expression and localization of Oct-1 by immunocytochemistry in mouse mammary gland and HC11 cells.

The sections of mammary tissue from a mid-lactating mouse (A–D) and HC11 cells (E, F) were incubated with either normal rabbit IgG (D) or anti-Oct-1 (A–C, E, F). In (C), the anti-Oct-1 antibody was pre-incubated with corresponding control peptide before applying to the tissue section. The immunostaining was detected by fluorochrome-coupled secondary antibodies and analysed with a confocal microscope. In (B, F), the tissue section or cells were counterstained with SYTOX nuclear stain.