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Calmodulin, calbindin D28k and secretagogin were immobilized in different flow cells on the same chip. SNAP-25 (1.5 μM) was first passed over calmodulin, then calbindin D28k and finally secretagogin. The flow buffer contained either 2 mM CaCl2 or 3.4 mM EDTA. The signal intensity before injecting SNAP-25 has been subtracted from all curves. RU, resonance units.
