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. 2006 Dec 11;401(Pt 1):309–316. doi: 10.1042/BJ20060880

Figure 4. Effects of NKY80, MDL-12,330A, tyrphostin A25 and SQ22536 on ACA, ACB and ACG activities.

Figure 4

(A–D) Cell lysates. For assay of ACB, ACG or ACA activity, aca–/rdeA–, aca–/A15::ACG or NC4 cells were lysed without or with 30 μM GTP[S] (NC4) and incubated with the indicated concentrations of NKY80 (A), MDL-12,330A (B), tyrphostin A25 (C) or SQ22536 (D). Accumulated cAMP levels were determined after 0 min (open symbols), 5 min (closed symbols, ACA, ACG) or 30 min (closed symbols, ACB). Data were standardized on control cAMP levels. (E, F) Intact cells. aca–/rdeA–, aca–/A15::ACG or NC4 cells were incubated with DTT and/or DcAMP/DTT in the presence of the indicated concentrations of tyrphostin A25 or SQ22536. Accumulated cAMP levels were determined after 0 min (open symbols), 3 min (ACA), 5 min (ACG) or 30 min (ACB). Data were standardized on levels obtained in the absence of inhibitor, and in case of ACA in the presence of DcAMP. Means±S.E.M. for two or three experiments performed in triplicate are presented.