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●, WT acetate/acetic; ○, H102A acetate/acetic; ▲, WT Mes/NaOH; △, H102A Mes/NaOH; ■, WT Hepes/HCl; (□) H102A Hepes/HCl; ▼, WT glycine/NaOH; ▽, H102A glycine/NaOH.
