Table 1. Kinetic parameters of the R5P to Ru5P and Ru5P to R5P isomerizations catalysed by wild-type (WT) and mutant TcRpiBs.

ND, not detectable; −, not determined.

	R5P → Ru5P		Ru5P → R5P
	Km (mM)	kcat (s−1)	Km (mM)	kcat (s−1)
WT	4±1	12±5	1.4±1.3	4.7±1.5
C69A	ND	ND	ND	ND
H102A	2±1	1	2.5±0.7	10.7±3.4
H138A	10±3	10±3	−	−
H11A	23±1	97±16	−	−