Figure 6. GPI anchor lipids of WT and mutant 4Δ.Lass5 cells are similar.

Labelling of 25 A₆₀₀ units of YPK9 (WT), 4Δ.Lass5, 2Δ.Lass5, 4Δ.Lass5 elo3Δ (lane 15) and 4Δ.Lass5 lip1Δ (lane 16) cells for 2 h with 100 μCi of [³H]inositol. Free lipids were extracted, GPI proteins were completely delipidated and their lipid moieties were liberated using HNO₂, with the exception of lane 13. Free lipids (L, lanes 1, 6–9 and 12) and liberated anchor lipids (A, lanes 2–5, 10, 11 and 13–16) were treated with NaOH (+) or mock incubated (−) for deacylation, separated by TLC and exposed for fluorography. IPCs were annotated as in Figure 3(A).