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. 2006 Dec 11;401(Pt 1):205–216. doi: 10.1042/BJ20061128

Figure 7. Phenotypic analysis of 4Δ.Lass5 cells under various stress conditions.

Figure 7

Serial 10-fold dilutions of cells were plated on buffered (A and D) or unbuffered YPD + uracil + adenine plates containing the indicated ingredients. Plates were then incubated at 30 °C and photographed after 3 days except for panel (D), where they were grown at 24 °C for 3 days. 4Δ.LAG1 cells were grown in YPG before being plated on YPD, where they strongly reduce sphingolipid biosynthesis but, nevertheless, continue to grow for several days. The vma4Δ cells served as a positive control and exhibited the same Vma− phenotype as described for vma2Δ and vma13Δ [23].