Table 2. Yeast saccharomyces cerevisiae strains.
Strains | Genotype | Reference |
---|---|---|
W303-1A | MATa can1-100 ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 | [11] |
YPK9 | MATa ade2-101ochre his3-Δ200 leu2-Δ1 lys2-801ambertrp1-Δ63 ura3-52 | [11] |
YPK11 | MATα ade2-101ochre his3-Δ200 leu2-Δ1 lys2-801ambertrp1-Δ63 ura3-52 | Michal Jazwinski |
2Δ.LAG1 | Same as YPK9 but lag1::TRP1 lac1::LEU2 containing pBM150:LAG1* | [11] |
4Δ.LAG1 | Same as YPK9 but lag1::TRP1 lac1::LEU2 | This study |
ydc1::natMX ypc1::kanMX4 containing pBM150:LAG1* | ||
4Δ.Lass5 | Same as 4Δ.LAG1 but containing p413MET:Lass5 instead of pBM150:LAG1* | This study |
4Δ.Lass5-ADE2 | Same as YPK9 but lag1::TRP1 lac1::LEU2 | This study |
ydc1::natMX ypc1::kanMX4::ADE2 containing p413MET:Lass5 | ||
4Δ.Lass5 elo3Δ | Same as 4Δ.Lass5-ADE2 containing elo3::kanMX | This study |
2Δ.Lass5 | Same as YPK9, but MATα lac1Δ::LEU2 | This study |
lag1Δ::TRP1 and containing p413MET:Lass5 | ||
4Δ.Lass5-URA3 | Same as YPK9 but lag1::TRP1 lac1::LEU2 | This study |
ydc1::natMX ypc1::kanMX4::URA3 containing p413MET:Lass5 | ||
4Δ.Lass5 lip1Δ | Same as 4Δ.Lass5-URA3 but lip1::kanMX4 | This study |
lcb1-100 | MATa end8-1 leu2 ura3 his4 bar1 | R. Schneiter |
4R3 | lcb1::URA3 SLC1-1 ura3-52 leu2-3,112 ade1 MEL1 | R. Dickson |
SJ21R | ura3-52 leu2-3,112 ade1 | K. Athenstaedt |
BY4742 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | EUROSCARF |
vma4Δ | YOR332w::kanMX4 in BY4742 | EUROSCARF |
elo3Δ-1 | YLR372w::kanMX4 in BY4742 | EUROSCARF |
elo3Δ-2 | YLR372w::his5+ in YPK11 | This study |
ypc1Δ ydc1Δ | Same as YPK9 but ypc1::kanMX4 ydc1::kanMX4::natMX | This study |
sec18 | MATα sec18ts | Howard Riezman |
*pBM150 is a CEN-ARS, URA3 vector possessing the yeast GAL1,10 promoter.