Table 3. Incorporation of [3H]inositol into sphingolipids.
The experiment shown in Figure 3(A) was performed two to seven times and the radioactivity of sphingolipids (as percentage of total labelled lipids) and of individual IPC types (as percentage of total IPCs) were calculated on the basis of radioscans of the TLC plates.
| Strain | No. of experiments | Sphingolipids (% of lipids) | IPC2 (% of IPCs) | IPC3 (% of IPCs) | IPC4 (% of IPCs) |
|---|---|---|---|---|---|
| 2ΔLass5 | 2 | 22.6±7.2 | 46.9±3.5 | 36.8±1.4 | 16.3±2.2 |
| 4ΔLass5 | 5 | 11±4.1 | 46.3±2.6 | 42.2±2.8 | 11.5±1.8 |
| 4ΔLass5 elo3Δ | 5 | 13.8±4.7 | 46.9±5.2 | 38.9±5.2 | 14.2±2.2 |
| 4ΔLass5 lip1Δ | 5 | 10.3±2.3 | 50.9±2.2 | 38.5±2.0 | 10.5±1.8 |
| YPK9 | 7 | 64.4±11.9 | - | - | - |