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. 2006 Dec 11;401(Pt 1):247–256. doi: 10.1042/BJ20061091

Figure 9. Regulation of HBx interaction with the cyclin–cdk complex by different signalling pathways.

Figure 9

X0-transfected Huh7 cells were grown in serum-free or serum-plus (10%) medium for 48 h and treated either with PD98059 (A), wortmannin (A) or genistein (B, C) for indicated time periods to inhibit endogenous MAPK, PI3K–Akt and Src kinases respectively. Cell extracts were immunoprecipitated with anti-HBx mAb followed by Western blotting for cyclin E (A, B). Extracts from the genistein-treated cells were also immunoprecipitated with anti-Src kinase antibody followed by Western blotting for phosphotyrosine (C). One-tenth of the cell extracts were also probed for the levels of active and total ERK1/2 and PI3K–Akt. The asterisk indicates treatment with wortmannin for 5 min. PI, preimmune control.