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. 2006 Dec 11;401(Pt 1):257–267. doi: 10.1042/BJ20060242

Figure 3. The C-terminus of SOCS1 and SOCS3 is not essential for signalling inhibition.

Figure 3

Inhibition of EpoR (A) and LR (B) signalling by SOCS1/SOCS3 mutants. HEK-293T cells were transiently co-transfected with plasmids encoding different LR tyrosine mutants or EpoR, various mutants of SOCS1 and SOCS3 and with the pXP2d2-rPAPI-luciferase reporter. The LR tyrosine mutants were used to minimize interference of other inhibitors. The transfected cells were either stimulated for 24 h with ligand or left untreated (NS). Luciferase measurements were performed in triplicate. Data are expressed as the stimulated/NS ratio+S.D.