Figure 5. .
μCT analysis of the femur. A, Volumetric reconstructions of cortical and cancellous bone from the metaphysis of wild-type and mutant distal femurs. The cortex from Ank null mice was slightly thinner but similar in shape, whereas the cancellous bone was sparse compared with wild type. B, Quantified values for images in panel A for mice euthanized at age 6 wk and 6 mo. Ank null mice exhibited decreased metaphyseal cortical thickness at age 6 wk but not at age 6 mo. In contrast, these mice exhibited a decreased ratio of bone:tissue volume (BV:TV) relative to wild type in the metaphyseal cancellous bone at both age 6 wk and age 6 mo. Note that the presence of BAC-AnkG389R had no affect on wild-type or Ank null mice. However, this BAC had a significant dominant negative effect on the cancellous region of +/Anknull mice at age 6 mo. A single asterisk (*) indicates P<.05; a double asterisk (**) indicates P<.01; a triple asterisk (***) indicates P<.001 versus wild-type control; a pound sign (#) indicates P<.01 for BAC+ versus BAC−; the number of mice analyzed is indicated at the base of each bar.