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. 2005 Sep;167(3):869–877. doi: 10.1016/S0002-9440(10)62058-5

Figure 2.

Figure 2

IRS-2 overexpression in HCC from DEN-injected and ASV mice. Protein extracts (40 μg) prepared from HCC (T) and nontumoral (NT) tissues from 12- and 13-month-old DEN-injected mice (A) and from 6-month-old ASV males (ASV 1 to 6) (B) were analyzed by Western blotting for IRS-2 protein expression. The liver was entirely tumoral in 6-month-old ASV males and the normal liver from an age-matched female was used as a control. Blots were reprobed with an anti-Akt antibody to ensure equivalent protein loading. Quantitative values are means ±SEM; *P < 0.05, compared with nontumoral tissue. Total RNA (2 μg) extracted from HCC (T) and nontumoral (NT) tissues from 12- and 13-month-old DEN-injected mice (C) and from 6-month-old ASV males (ASV 1 to 3) (D) and a control female were analyzed by semiquantitative RT-PCR using murine IRS-2 primers. β-Actin PCR products were run in parallel to ensure that equivalent amounts of cDNA were amplified.