Figure 4.

Immunohistochemical detection of IRS-2 in the liver from control and DEN-injected mice. Liver tissue sections from control and DEN-injected mice were immunolabeled with an anti-IRS-2 antibody (reddish-brown labeling) and counterstained with hematoxylin. Histologically normal liver from a 3-month-old noninjected male (A) and from a 3-month-old DEN-injected male (B). IRS-2 immunoreactivity is detected in isolated hepatocytes (arrowheads) and in a small cluster (arrows). C: HCC nodules from a 12-month-old DEN-injected male. Original magnifications, ×200.