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. 2005 Sep;167(3):869–877. doi: 10.1016/S0002-9440(10)62058-5

Figure 8.

Figure 8

IRS-2 overexpression in human HCC specimens. Protein extracts and total RNA were prepared from human HCC (T) and nontumoral (NT) liver tissues from seven patients. A: IRS-2 and IRS-1 protein expressions were evaluated by Western blotting analysis. Blots were reprobed with an anti-Akt antibody to ensure equivalent protein loading. B: IRS-2 mRNA expression was evaluated by semiquantitative RT-PCR. β-Actin PCR products were run in parallel to ensure that equivalent amounts of cDNA were amplified.