Table 1.
Expression of Different Components of the Insulin/IGF Signaling Axis in DEN-Induced Liver Tumors with IRS-2 Overexpression
| Molecules | Overexpression frequency |
|---|---|
| IGF-2 | 8 of 11 (73%) |
| Insulin receptor | 4 of 16 (25%) |
| IGF-1R | 0 of 16 |
| IRS-1 | 13 of 17 (76%) |
| p-Ser9GSK-3β | 12 of 17 (70%) |
Eighty-five percent of DEN-induced liver tumors exhibited IRS-2 overexpression and were examined for insulin receptor, IGF-1R, IRS-1, and phospho-Ser9 GSK-3β expression by Western blotting analysis and for IGF-2 mRNA expression by RT-PCR.