Figure 3.

Altered expression levels of hMC DNA, protein, and secreted protein in response to PDGF ± LXA₄, validating changes demonstrated by Affymetrix microarray. hMCs were treated with PDGF (10 ng/ml), LXA₄ (1 nmol/L), vehicle, or LXA₄ (15 minutes) followed by PDGF. a: Changes in the expression of collagen I αI and fibronectin cDNA after 24 hours, as measured by real-time PCR. The average of two independent experiments performed in duplicate is shown. b: Altered levels of fibronectin, thrombospondin 1, and decorin protein measured by immunoblotting after 24 hours, corresponding β-actin blots demonstrate equal loading. Data are representative of three independent experiments. c: TGF-β₁ and MMP-1 secretion from hMCs as measured by ELISA at the indicated time points. Data shown are mean ± SEM of duplicate measurements from three independent experiments (*P < 0.05, **P < 0.005).