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JAK2 mutation analysis in patients with IM (A), PV (B), or ET (C). The presence of the Val617Phe JAK2 mutation was analyzed by an allele-specific PCR; the 203-bp product is specifically amplified from the mutant allele, while the 364-bp product serves as an internal control for both mutant and normal allele. The percentage of GATA-1neg megakaryocytes according to the presence or not of JAK2 mutation is reported in D.
