Table 2.

Primers Used for PCR Analysis

GATA-1 forward primer: 5′ CAAGAAGCGCCTGATTGTCAG 3′

GATA-1 reverse primer: 5′ AGTGTCGTGGTGGTCGTCTG 3′

GATA-1 probe: 5′ AAACGGGCAGGTACTCAGTGCACCAACT 3′

Amplified fragment size: 71 bp

GATA-2 forward primer: 5′ ATCCAGACTCGGAACCGGA 3′

GATA-2 reverse primer: 5′ GCACTTTGACAGCTCCTCGAA 3′

GATA-2 probe: 5′ ATGTCCAACAAGTCCAAGAAGAGCAAGAAAGG 3′

Amplified fragment size: 83 bp

FOG-1 forward primer: 5′ TTGCCACCGCAGTGATCA 3′

FOG-1 reverse primer: 5′ GCTCGCTGCGGTACCAGAT 3′

FOG-1 probe: 5′ AGACGTCTTCCCCTGCAAGGACTGTG 3′

Amplified fragment size: 68 bp

GATA-1 alternative transcripts36

Exon 1 forward primer: 5′ AGGACACCCCCTGGGATC 3′

Exon 3 reverse primer: 5′ CTCCATACAGTTGAGCAATGGG 3′

Amplified fragment size (full-length cDNA): 384 bp

Amplified fragment size (alternative splice variant cDNA): 109 bp

JAK2 mutation analysis16

Reverse primer: 5′ CTGAATAGTCCTACAGTGTTTTCAGTTTCA 3′

Forward (specific): 5′ AGCATTTGGTTTTAAATTATGGAGTATATT 3′

Forward (internal control): 5′ ATCTATAGTCATGCTGAAAGTAGGAGAAAG 3′