Figure 2.

Differential expression of PDGF receptors and ligands in mouse hematopoietic lineages. Thioglycollate-elicited peritoneal macrophages, BMDMs, and splenocytes were prepared from wild-type male C57BL/6J mice. B cells and T cells were purified from total splenocytes by negative selection as described in Materials and Methods. Mouse aortic cells (primarily smooth muscle) were isolated from newborn mouse aorta and used as a positive control. Total RNA was purified from each cell population and treated with DNase to remove contaminating DNA. Two hundred ng of purified RNA was used in a One-Step RT-PCR reaction with specific primer pairs as indicated in Table 1 and the PCR products were run on a 1% agarose gel using L32 and GAPDH as controls for RNA load. Comparable expression levels were observed in hematopoietic cells from ApoE−/− mice (data not shown).