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. 2005 Sep;167(3):787–796. doi: 10.1016/s0002-9440(10)62051-2

Figure 2.

Figure 2

Effect of IL-12 and TGF-β1 on FT-IV, FT-VII, and C2GnT mRNA in eosinophils. Isolated human eosinophils were stimulated with IL-12 (10 ng/ml) or TGF-β1 (5 ng/ml) for 18 hours. A and B: Gel electrophoresis for PCR products at various PCR cycles. C: Real-time PCR analysis. Relative quantity indicates the relative amount of mRNA of target genes compared with that of β-actin mRNA (n = 3). Relative quantity of genes in unstimulated cells (medium alone) was regarded as 1.0. IL-12 enhances C2GnT mRNA expression in eosinophils, whereas FT-IV and FT-VII mRNA levels were up-regulated by TGF-β1. C2GnT mRNA was weakly enhanced by TGF-β1, but this was not statistically significant. *P < 0.05 compared with corresponding sample incubated in medium alone.