Figure 10.

Progression of proteinuria is attenuated in Alport mice treated with either MMI-270 or propagermanium compared to untreated Alport mice. Urine was collected from mice weekly at the indicated time points and frozen. Equivalent volumes were fractionated on acrylamide gels, and the gels were stained with Coomassie Blue. Images were captured using a Viewsonic image analysis system, and the bands were quantified densitometrically using LabWorks software (UVP, Inc., Upland, CA). Data are presented as means with standard deviations for triplicate samples. Animals from both MMI 270- and propagermanium-treated groups survived through approximately 12 weeks of age. Asterisks denote statistically significant differences for proteinuria when comparing Alport mice with either MMI-270 or propagermanium-treated Alport mice (P > 0.005).