Figure 6.

Expression of both MCP-1 and CCR2 are induced in glomeruli from Alport mice relative to wild-type mice. A and B: RNA from isolated glomeruli (A, lanes 3 and 4; B, lanes 1 and 2) or cultured glomerular podocytes (A, lanes 1 and 2) was amplified using primers specific for CCR2 (A) or MCP1 (B). GAPDH was amplified as a control. A, Alport; C, wild-type. C: Western blot analysis of protein from cultured wild-type mesangial cells (MC), cultured wild-type podocytes (podo), or isolated glomeruli (glom) from wild-type (Wt) and Alport (Alp) mice probed with antibodies specific for CCR2. One-tenth of the protein was fractionated on a duplicate gel and stained with Coomassie Blue to control for loading (total protein).