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. 2006 Jul;169(1):189–199. doi: 10.2353/ajpath.2006.051225

Figure 5.

Figure 5

A: The amount of IFN-γ mRNA in the cells isolated from the spinal cord of uninfected rats was quantified by real-time RT-PCR. Samples were obtained from the cells after treatment with recombinant IL-12 (100 ng/ml) for 18 hours (black columns). Results of experiments done in triplicate and repeated three times were evaluated as mean ± SE values of the fold increase to the data without IL-12 treatment (white columns). *P < 0.05. B: Cells isolated from the spinal cord of uninfected rats were cultured on poly-l-lysine/laminin-coated glasses. After incubation with recombinant IL-12 (100 ng/ml) for 5 days, immunofluorescent double staining was performed using anti-IFN-γ (green) and anti-NF (red) antibodies. Representative merged images are shown. Experiments were performed independently three times. C: The amount of IL-12Rβ1 and IL-12Rβ2 mRNAs in the cells isolated from the spinal cord of uninfected rats was quantified by real-time RT-PCR. Samples were obtained from the cells after treatment with recombinant IL-12 (100 ng/ml) for 18 hours (black columns). Results of experiments done in triplicate and repeated three times were evaluated as mean ± SE values of the fold increase to the data without IL-12 treatment (white columns). *P < 0.05. For all experiments, at least three rats were used in each group. Original magnifications, ×620 (B).