Figure 5-6913.
A: FTC-133-RLN2 transfectants (gray columns) displayed significantly (asterisk) increased NADH2-dependent production of formazan salts after 6-hour incubations compared to FTC-133-EGFP controls (black columns). B: Also reflecting enhanced mitochondrial activity, increased intracellular ATP levels were determined in the FTC-133-RLN2 clones at all cell densities investigated. Nonradioactive BrdU proliferation assays of FTC-133 transfectants revealed a small but significant (asterisk) increase in proliferation rates by FTC-133-RLN2 when seeded at lower cell numbers (0.5 × 104 cells with P ≤ 0.005). A statistically less significant (P ≤ 0.01) increase in proliferation was observed with 0.25 × 104 cells per well. C: The proliferative response to relaxin was diminished at high cell numbers (104 cells) indicating that RLN2 did not act as a strong mitogen on FTC-133. All data from these three assays were normalized to 0.5 × 104 cells.