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Differential methylation in tissue development. Real RLGS profiles from C57BL/6J liver and C57BL/6J kidney genomic DNA. The spot indicated by the arrow is very faint in liver genomic DNA. The spot DNA sequence was confirmed by PCR of spot DNA eluted from the kidney genomic DNA gel using primers determined from the virtual sequence. Bisulfite sequencing of the liver genomic DNA indicates that the two CpGs at NotI sites and two adjacent sites are highly methylated (underlined).
