Figure 8-6943.

MMP-2 activity. Supernatants of MCs isolated from mice on placebo or CE₂ were collected for zymography as described in Materials and Methods. A: Representative zymogram of supernatants of MCs that were treated with vehicle (V), E₂ (0.1 and 1 nmol/L), ICI (1 μmol/L), or ICI + E₂ (1 nmol/L). B: Graph depicting the mean ± SEM of duplicate experiments of MMP-2 activity in the supernatant of MCs isolated from mice on placebo after in vitro E₂ stimulation. C: Graph depicting the mean ± SEM of duplicate experiments of MMP-2 activity after in vitro E₂ stimulation of MCs isolated from mice on CE₂. Data are expressed as percentage of vehicle control. *P < 0.05.