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. 2006 Aug;169(2):599–616. doi: 10.2353/ajpath.2006.060002

Table 1.

Antibodies and Lectin Used in This Study

Antibody Species Specificity Dilution Source
AT8 Mouse Tau; pSer202/pThr205 1:400 (WB); 1:10,000 (ICH/IF) Innogenetics (Gent, Belgium)
pSer396 Rabbit Tau; pSer396 1:5000 Biosource (Camarillo, CA)
AT180 Mouse Tau; pThr231 1:500 Innogenetics
AT270 Mouse Tau; pThr181 1:4000 (WB); 1:2000 (IHC) Innogenetics
AP422/988 Mouse Tau; pSer422 1:5000 Bussiere et al61
AT100 Mouse Tau; pThr212/pSer214 1:1000 (WB); 1:2000 (IHC) Innogenetics
M19G Rabbit Tau; human-specific, 1 to 19 amino acids 1:20,000 Bussiere et al61
MC1 Mouse Tau; conformational epitope, 7 to 9 and 326 to 330 amino acids 1:1000 P. Davies
PHF-1 Mouse Tau; pSer396/pSer404 1:50,000 P. Davies
Tau-5 Mouse Tau; human/mouse-specific 1:10,000 Biosource
TP20 Rabbit Tau; human-specific, 33 to 41 amino acids 1:2000 Brion et al15
12E8 Mouse Tau; pSer262 1:400 Seubert et al62
GFAP Rabbit GFAP 1:20,000 (IF) DAKO (Trappes, France)
Synaptophysin Rabbit Synaptophysin 1:1000 Santa Cruz Biotechnology (Santa Cruz, CA)
Synaptotagmin Rabbit Synaptotagmin I 1:1000 Synaptic System (Göttingen, Germany)
Lectin
Specificity
Dilution
Source
Biotinylated tomato lectin (Lycopersicon esculentum) Microglia 6 μg/ml Sigma-Aldrich L-0651 (Sigma-Aldrich, Lyon, France)