Figure 2-6916.

CNTF does not modify T-cell proliferation and cytokine mRNA levels in response to immunization with MOG peptide. A: Lymph nodes were collected from mice immunized with MOG plus complete Freund’s adjuvant and treated with 180 μg/kg/day (n = 8), 1.2 mg/kg/day CNTF (n = 7), or saline (n = 11) at days 15 to 22 after immunization. Proliferative response of isolated LN cells was assayed in vitro. Stimulation with 50 μg of MOG(35-55) peptide led to a marked MOG-dependent proliferation that was not altered by CNTF treatment. B and C: Cytokine mRNA levels were assessed by real-time PCR analysis of RNA isolated from lymph nodes or spleen. The results for 180 μg/kg/day (n = 9) and 1.2 mg/kg/day CNTF (n = 2) were derived from two separate experiments. Results are shown within one graph with the appropriate saline control groups (n = 9 or 6, respectively). B: The levels of IFN-γ mRNA did not differ significantly between CNTF-treated and saline-injected mice. C: Mice treated with 1.2 mg/kg/day CNTF had significantly higher levels of IL-10 mRNA. D–F: Levels of IL-2, IFN-γ, and IL-5 protein in the supernatants of lymph node cell cultures from mice treated with saline (n = 8) or 1.2 mg/kg/day CNTF (n = 6) were measured by ELISA. No significant differences were detected.